JOVE JOURNAL

Biochimica

Showing 397 - 408 in 778 videos.

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Karine Nozeret¹, Aurélia Pernin¹, Nienke Buddelmeijer¹

¹Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France

Journal (Biochemistry)

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Stuart W. McKellar¹, Ivayla Ivanova¹, Robert W. van Nues², Ross A. Cordiner³, Mehak Chauhan¹, Niki Christopoulou¹, Will Worboys⁴, Andrew Langford⁴, Torben Heick Jensen³, Sander Granneman¹

¹Centre for Engineering Biology,University of Edinburgh, ²Institute of Cell Biology,University of Edinburgh, ³Department of Molecular Biology and Genetics,Aarhus University, ⁴UVO3 Ltd.

Journal (Biochemistry)

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

Monica C. Pillon¹, Robin E. Stanley¹

¹Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Journal (Biochemistry)

A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes

Yeganeh Habibi¹, Christopher J. Thibodeaux¹

¹Department of Chemistry,McGill University

Journal (Biochemistry)

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Dohwan Lee*¹, Kyung Wook Wee*¹, Hyerin Kim*¹, Sung Il Han¹, Seungmin Kwak^1,2, Dae Sung Yoon³, Jeong Hoon Lee¹

¹Department of Electrical Engineering,Kwangwoon University, ²Korea Institute of Science and Technology (KIST), ³School of Biomedical Engineering,Korea University

Journal (Biochemistry)

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Qin Fu¹, Casey W. Johnson¹, Bhagya K. Wijayawardena², Michael P. Kowalski², Miranda Kheradmand², Jennifer E. Van Eyk¹

¹Advanced Clinical Biosystems Institute, Smidt Heart institute,Cedars Sinai Medical Center, ²Beckman Coulter Life Sciences

Journal (Biochemistry)

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Ritika Tewari*¹, Savannah J. West*^1,2, Bieerkehazi Shayahati¹, Askar M. Akimzhanov¹

¹Department of Biochemistry and Molecular Biology,McGovern Medical School at UT Health, ²MD Anderson UT Health Graduate School

Journal (Biochemistry)

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

Jessica A. Espino¹, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences,University of Maryland

Journal (Biochemistry)

Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins

David J. Speca¹, Elva Diaz¹

¹Department of Pharmacology,UC Davis School of Medicine

Journal (Biochemistry)

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Maria Lucia Pigazzini*^1,2, Christian Gallrein*¹, Manuel Iburg*¹, Gabriele Kaminski Schierle³, Janine Kirstein^1,4

¹Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, ²NeuroCure Cluster of Excellence,Charité - Universitätsmedizin Berlin, ³Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology,University of Cambridge, ⁴Cell Biology,University of Bremen

Journal (Biochemistry)

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Alexandria Slayden¹, Kelsey North¹, Shivantika Bisen¹, Alex M. Dopico¹, Anna N. Bukiya¹, Avia Rosenhouse-Dantsker²

¹Department of Pharmacology, Addiction Science and Toxicology,The University of Tennessee HSC, ²Department of Chemistry,University of Illinois at Chicago

Journal (Biochemistry)

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Karel Bezstarosti¹, Lennart van der Wal¹, Jeroen A. A. Demmers¹

¹Proteomics Center,Erasmus University Medical Center

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