需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Generating Genetically Modified Plasmodium berghei Sporozoites
Chapters
- 00:04Introduction
- 00:57Generation of Schizonts in Culture
- 02:25Transfection of Plasmodium Schizonts
- 04:58Selection of the Transfected Parasites
- 05:52Infection of Mosquitoes with the Transgenic Lines
- 07:15Collection of Sporozoites
- 08:53Results: Detecting the Lysis of Plasmodium and its Parasitophorous Vacuole in Host Hepatocytes
- 09:42Conclusion
Malaria is transmitted through inoculation of the sporozoite stage of Plasmodium by infected mosquitoes. Transgenic Plasmodium has allowed us to understand the biology of malaria better and has contributed directly to malaria vaccine development efforts. Here, we describe a streamlined methodology to generate transgenic Plasmodium berghei sporozoites.
