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Isolamento de núcleos de tecido hepático congelado por flash para multiômica unicelular
JoVE 杂志
生物学
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JoVE 杂志 生物学
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Isolamento de núcleos de tecido hepático congelado por flash para multiômica unicelular

4,396 Views

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09:09 min

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December 09, 2022

DOI:

10.3791/64792-v

DOI:
10.3791/64792-v

•

09:09 min
December 09, 2022

•

75 Views
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1Helmholtz Pioneer Campus (HPC),Helmholtz Munich, 2Institute of Computational Biology, Computational Health Department,Helmholtz Munich, 3Division of Infectious Diseases and Tropical Medicine,Ludwig-Maximillian-Universität Klinikum, 4TUM School of Medicine,Technical University of Munich

Summary

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Aqui, apresentamos um protocolo para isolar núcleos de tecidos hepáticos arquivados congelados por flash para RNA-seq, ATAC-seq de núcleo único e multiômica articular (RNA-seq e ATAC-seq).

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Cite this Article

Strzelecki, M., Yin, K., Talavera-López, C., Martinez-Jimenez, C. P. Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics. J. Vis. Exp. (190), e64792, doi:10.3791/64792 (2022).

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