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Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research

Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research

2,719 Views

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08:20 min

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May 12, 2023

DOI:

10.3791/64586-v

DOI:
10.3791/64586-v

•

08:20 min
May 12, 2023

•

5 Views
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1Department of Applied Stem Cell Technologies, TechMed Centre Enschede,University of Twente, 2Centro de Biotecnología-FEMSA,Tecnologico de Monterrey, 3Department of Genetics,Harvard Medical School

Summary

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The present protocol provides a step-by-step procedure for the reproducible generation, maintenance, and aging of cerebral organoids derived from human-induced pluripotent stem cells (iPSCs). This method enables culturing and maturing cerebral organoids for extended periods, which facilitates the modeling of processes involved in brain aging and age-related pathogenesis.

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Koch, L. S., Choy Buentello, D., Broersen, K. Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research. J. Vis. Exp. (195), e64586, doi:10.3791/64586 (2023).

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