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评估半胱天冬酶活化以评估先天免疫细胞死亡
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
DOI:

10:23 min

January 20, 2023

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Chapters

  • 00:05Introduction
  • 01:04Stimulating the Cells
  • 02:05Collecting the Combined Supernatant and Protein Lysate to Be Used for Caspase Western Blots
  • 03:20Performing Western Blotting Using the Lysates Collected from the BMDMs or Tissue Homogenates
  • 07:55Results: Monitoring Caspase Cleavage and Activation
  • 09:31Conclusion

Summary

自动翻译

该协议描述了一种评估半胱天冬酶活化(半胱天冬酶-1,半胱天冬酶-3,半胱天冬酶-7,半胱天冬酶-8,半胱天冬酶-9和半胱天冬酶-11)的综合方法,以响应 体外 体内 (小鼠)感染,无菌侮辱和癌症模型,以确定细胞死亡途径的启动,例如焦亡,细胞凋亡,坏死性凋亡和全细胞凋亡。

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