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Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
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JoVE 杂志 化学
Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

DOI:
10.3791/64293-v

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10:12 min

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September 19, 2022

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, , ,
1Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering,Southwest Jiaotong University

Chapters

  • 00:04Introduction
  • 01:10Equipment Preparation
  • 01:44Synthesis of FITC‐Labeled Linear R8 Peptide and FITC‐Labeled Stapled R8 Peptide
  • 05:49Exploration of the Cell‐to‐Cell Penetration of the Peptides Using Transwell Models
  • 07:06Results: Synthesis of FITC‐R8 and FITC‐sR8‐4 Using Solid‐Phase Peptide Synthesis and Evaluation of Their Trans‐Barrier Permeability
  • 09:06Conclusion

Summary

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This protocol describes the synthesis of cyclic cell-penetrating peptides with aromatic cross-links and the evaluation of their permeability across biological barriers.

Tags

Cyclic Cell-penetrating PeptidesBiological BarriersCancer TreatmentMolecular TransportersDrug DeliveryPeptide CyclizationAromatic Cross-linksHydrophobicityManual Peptide SynthesisFmocHATUDIPEA

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