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冷冻电子断层扫描远程数据收集和断层扫描平均

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

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冷冻电子断层扫描远程数据收集和断层扫描平均

4,103 Views

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08:55 min

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July 12, 2022

DOI:

10.3791/63923-v

DOI:

10.3791/63923-v

•

08:55 min

July 12, 2022

•

15 Views

Yuewen Sheng¹, Kyle Morris¹, Julika Radecke*¹, Peijun Zhang*^1,2,3

¹Electron Bio-Imaging Centre,Diamond Light Source Ltd, Harwell Science & Innovation Campus, ²Division of Structural Biology, Wellcome Trust Centre for Human Genetics,University of Oxford, ³Chinese Academy of Medical Sciences Oxford Institute,University of Oxford

Summary

本协议描述了使用Tomo5的高分辨率冷冻电子断层扫描远程数据采集以及使用emClarity的后续数据处理和子断层图平均。以载铁蛋白为例，说明实现 2.86 Å 分辨率冷冻电子断层扫描结构的详细分步过程。

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