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Establishment of Zebrafish Patient-Derived Xenografts from Pancreatic Cancer for Chemosensitivity Testing
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JoVE Journal Cancer Research
Establishment of Zebrafish Patient-Derived Xenografts from Pancreatic Cancer for Chemosensitivity Testing

Establishment of Zebrafish Patient-Derived Xenografts from Pancreatic Cancer for Chemosensitivity Testing

DOI:
10.3791/63744-v

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06:59 min

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May 12, 2023

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, , , ,
1Department of Biology,University of Pisa, 2General Surgery Unit, Department of Translational Research and New Technologies in Medicine and Surgery,University of Pisa

Chapters

  • 00:04Introduction
  • 00:56Sample Processing
  • 03:11Establishment of Zebrafish‐Based Patient‐Derived Xenografts (zPDX)
  • 04:56Treatment and Imaging
  • 05:40Results: Establishment of Zebrafish‐Based Patient‐Derived Xenografts (zPDXs) from Primary Human Pancreatic Adenocarcinoma
  • 06:14Conclusion

Summary

Automatic Translation

Automatic Translation

Preclinical models aim to advance the knowledge of cancer biology and predict treatment efficacy. This paper describes the generation of zebrafish-based patient-derived xenografts (zPDXs) with tumor tissue fragments. The zPDXs were treated with chemotherapy, the therapeutic effect of which was assessed in terms of cell apoptosis of the transplanted tissue.

Tags

Keywords: ZebrafishPatient-derived XenograftPDXPancreatic CancerChemosensitivity TestingTumor MicroenvironmentPersonalized MedicineCell StainingXenograft Establishment

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