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Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
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June 13, 2019
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We describe a detailed protocol for the isolation of tumor-initiating cells from human sarcoma patient-derived xenografts by fluorescence-activated cell sorting, using human leukocyte antigen-1 (HLA-1) as a negative marker, and for the further validation and characterization of these HLA-1-negative tumor-initiating cells.
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Han, D., Rodriguez-Bravo, V., Izadmehr, S., Domingo-Domenech, J., Cordon-Cardo, C. Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts. J. Vis. Exp. (148), e57011, doi:10.3791/57011 (2019).
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