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通过高分辨率呼吸测量法评估坐骨神经的线粒体功能
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生物化学
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JoVE 杂志 生物化学
Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
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通过高分辨率呼吸测量法评估坐骨神经的线粒体功能

2,136 Views

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08:19 min

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May 05, 2022

DOI:

10.3791/63690-v

DOI:
10.3791/63690-v

•

08:19 min
May 05, 2022

•

11 Views
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1Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde,Universidade Federal do Rio de Janeiro

Summary

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高分辨率呼吸测量与荧光传感器相结合，可测定线粒体耗氧量和活性氧 （ROS） 生成。本方案描述了一种评估透化坐骨神经中线粒体呼吸频率和ROS产生的技术。

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Formiga-Jr, M. A., Camacho-Pereira, J. Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry. J. Vis. Exp. (183), e63690, doi:10.3791/63690 (2022).

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