Journal
/
/
Deplezione extracellulare di glucosio come misura indiretta dell'assorbimento di glucosio nelle cellule e nei tessuti Ex Vivo
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
DOI:

10:35 min

April 06, 2022

, , , , ,

Chapters

  • 00:04Introduction
  • 01:043T3‐L1 Cell Culture and Maintenance
  • 01:49Starvation of 3T3‐L1 Cells
  • 02:22Preparation of FD‐Glucose Solutions with Different Concentrations
  • 04:19Treating Starved 3T3‐L1 Cells
  • 05:03Extracellular and Intercellular Measurements for Stimulated 3T3‐L1 Cells
  • 06:00Protein‐Based Normalization of Intercellular Glucose Uptake
  • 06:35Ex Vivo Measurement of Extracellular FD‐Glucose Depletion in Organs
  • 07:59Results: Extracellular FD‐Glucose Correlation with Intracellular Uptake and Kinetics of Extracellular FD‐Glucose Depletion
  • 09:39Conclusion

Summary

自动翻译

L'esaurimento extracellulare del glucosio marcato fluorescentemente è correlato all'assorbimento del glucosio e potrebbe essere utilizzato per lo screening ad alto rendimento dell'assorbimento del glucosio negli organi asportati e nelle colture cellulari.

Related Videos

Read Article