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Extracellulaire glucosedepletie als een indirecte maat voor glucoseopname in cellen en weefsels Ex Vivo
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
DOI:

10:35 min

April 06, 2022

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Chapters

  • 00:04Introduction
  • 01:043T3‐L1 Cell Culture and Maintenance
  • 01:49Starvation of 3T3‐L1 Cells
  • 02:22Preparation of FD‐Glucose Solutions with Different Concentrations
  • 04:19Treating Starved 3T3‐L1 Cells
  • 05:03Extracellular and Intercellular Measurements for Stimulated 3T3‐L1 Cells
  • 06:00Protein‐Based Normalization of Intercellular Glucose Uptake
  • 06:35Ex Vivo Measurement of Extracellular FD‐Glucose Depletion in Organs
  • 07:59Results: Extracellular FD‐Glucose Correlation with Intracellular Uptake and Kinetics of Extracellular FD‐Glucose Depletion
  • 09:39Conclusion

Summary

自动翻译

Extracellulaire depletie van fluorescerend gelabelde glucose correleert met glucoseopname en kan worden gebruikt voor high-throughput screening van glucoseopname in weggesneden organen en celculturen.

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