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Samodling av murina tunntarmsepitelorganoider med medfödda lymfoida celler
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JoVE 杂志 免疫与感染
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
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Samodling av murina tunntarmsepitelorganoider med medfödda lymfoida celler

4,666 Views

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08:22 min

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March 23, 2022

DOI:

10.3791/63554-v

DOI:
10.3791/63554-v

•

08:22 min
March 23, 2022

•

28 Views
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1Centre for Host-Microbiome Interactions,King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme,King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute,Cambridge University

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Co-cultureMurineSmall IntestineEpithelial OrganoidsInnate Lymphoid CellsIn Vitro ModelIntestinal HomeostasisInflammationILC1CD44Gastrointestinal DiseasesThermal Cross-linkingBasal Extracellular MatrixAdvanced DMEM F12PBS2Centrifugation

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Read, E., Jowett, G. M., Coman, D., Neves, J. F. Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells. J. Vis. Exp. (181), e63554, doi:10.3791/63554 (2022).

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