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Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
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JoVE 杂志 免疫与感染
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

DOI:
10.3791/63525-v

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08:30 min

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May 10, 2022

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, , , , , ,
1Otolaryngological Department of Tangdu Hospital,Fourth Military Medical University, 2Institute of Medical Research,Northwestern Polytechnical University, 3Orthopedic Department of Tangdu Hospital,Fourth Military Medical University, 4Department of Immunology,Fourth Military Medical University

Chapters

  • 00:05Introduction
  • 01:00Murine Allergic Rhinitis Model Establishment
  • 02:03Isolation of Mononuclear Cells from the Nasal Mucosa
  • 04:52Surface Staining for Flow Cytometry Analysis
  • 06:08Results: Ovalbumin-Induced Murine Model Development to Explore the Role of ILC2s in Allergic Rhinitis
  • 07:27Conclusion

Summary

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Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.

Tags

Keywords: Innate Lymphoid CellsILC2Nasal MucosaMouseFlow CytometryAllergic RhinitisOvalbuminCell Isolation

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