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Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination
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神经科学
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JoVE 杂志 神经科学
Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

DOI:
10.3791/63511-v

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04:53 min

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April 05, 2022

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, , , ,
1Department of Neurology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology

Chapters

  • 00:04Introduction
  • 00:42Mice Dissection and Tissue Dissociation
  • 01:35Debris Removal and Magnetic Separation of Microglial Cells
  • 03:26Results: Gating Strategy Used in Flow Cytometry Analysis Before and After MACS and Gating Strategy for Living/Dead Single Cells After MACS
  • 04:27Conclusion

Summary

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Here, we present a protocol to isolate and purify primary microglia in animal models of demyelinating diseases, utilizing columnar magnetic-activated cell sorting.

Tags

Mouse Primary MicrogliaMagnetic-activated Cell SortingDemyelinationCorpus CallosumEnzyme DigestionCD11b BeadsPositive SelectionMagnetic Field

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