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Chapters
- 00:04Introduction
- 00:56Creating a New CryoSPARC v3 Project and Importing Data
- 01:34CryoSPARC v3: Movie Alignment and Contrast Transfer Function (CTF) Estimation
- 02:52CryoSPARC v3: Manual and Template-Based Particle Picking
- 04:21CryoSPARC v3: 2D Classification
- 04:52CryoSPARC v3: Ab-initio Reconstruction and Homogeneous Refinement
- 05:31Exporting Particle Coordinates from CryoSPARC v3 and Importing Them to RELION-3 Using PyEM
- 05:56RELION-3: Particle Extraction and 2D Classification, 3D Refinement, Mask Creation, and Post-Processing
- 07:41RELION-3: Polishing Training, Particle Polishing, CTF, and Per-Particle Refinements
- 09:15Transferring RELION-3 Particle Coordinates and 3D Map to Scipion 3
- 10:05Scipion 3: High-Resolution Refinement
- 11:21Results: Using cryoSPARC v3, RELION-3, and Scipion 3 to Obtain a High-Resolution 3D Reconstruction of the Adeno-Associated Virus
- 12:52Conclusion
Este artigo descreve como utilizar efetivamente três plataformas de processamento crio-EM, ou seja, crioSPARC v3, RELION-3 e Scipion 3, para criar um fluxo de trabalho único e robusto aplicável a uma variedade de conjuntos de dados de partículas únicas para determinação da estrutura de alta resolução.
