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JoVE Journal Biochemistry
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
 

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Article DOI: 10.3791/63387-v • 13:43 min • January 31st, 2022
January 31st, 2022 •
1, 1,2
1Institute for Quantitative Biomedicine, Rutgers University, 2Department of Biochemistry & Microbiology, Rutgers University

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This article describes how to effectively utilize three cryo-EM processing platforms, i.e., cryoSPARC v3, RELION-3, and Scipion 3, to create a single and robust workflow applicable to a variety of single-particle data sets for high-resolution structure determination.

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Keywords: Cryo-EM Single-particle Analysis CryoSPARC RELION Scipion Adeno-associated Virus Gene Therapy Patch Motion Correction CTF Estimation Micrograph Curation Manual Particle Picking Template Generation Particle Extraction
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