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Chapters
- 00:05Introduction
- 01:22Induction of Definitive Endoderm Differentiation in Human Pluripotent Stem Cells (hPSCs) (Stage 1)
- 02:10Immunofluorescence Analysis of hPSC-Derived Definitive Endoderm (Stage 1)
- 04:19Generation of the Primitive Gut Tube from hPSCs (Stage 2)
- 05:50Assessment of Differentiation Efficiency of Generating Pancreatic Progenitors
- 08:10Results: Comparison between Optimized and Non-Optimized Protocol for Scalable Generation of Pancreatic Progenitors from hPSCs
- 09:21Conclusion
Denne protokollen beskriver en forbedret metode for å øke kouttrykket av PDX1- og NKX6.1-transkripsjonsfaktorer i pankreasprogenitorer avledet fra humane pluripotente stamceller (hPSCer) i plane monolag. Dette oppnås ved å fylle opp den friske matrisen, manipulere celletettheten og dissosiere de endodermale cellene.
