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Controle van celhechting met behulp van Hydrogel-patroontechnieken voor toepassingen in tractiekrachtmicroscopie
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
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Controle van celhechting met behulp van Hydrogel-patroontechnieken voor toepassingen in tractiekrachtmicroscopie

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12:26 min

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January 29, 2022

DOI:

10.3791/63121-v

DOI:
10.3791/63121-v

•

12:26 min
January 29, 2022

•

8 Views
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1Department of Cellular Biophysics,Max Planck Institute for Medical Research, 2Institute for Theoretical Physics and BioQuant,Heidelberg University, 3Institute for Molecular Systems Engineering (IMSE),Heidelberg University

Summary

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Near-UV-lithografie en tractiekrachtmicroscopie worden gecombineerd om cellulaire krachten op microgepatterde hydrogels te meten. De gerichte licht-geïnduceerde afgifte van enkele cellen maakt een groot aantal metingen op hetzelfde monster mogelijk.

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Christian, J., Blumberg, J. W., Probst, D., Lo Giudice, C., Sindt, S., Selhuber-Unkel, C., Schwarz, U. S., Cavalcanti-Adam, E. A. Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy. J. Vis. Exp. (179), e63121, doi:10.3791/63121 (2022).

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