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生物工程
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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
JoVE 杂志
生物工程
Author Produced
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JoVE 杂志 生物工程
Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

DOI:
10.3791/63121-v

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12:26 min

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January 29, 2022

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, , , , , , ,
1Department of Cellular Biophysics,Max Planck Institute for Medical Research, 2Institute for Theoretical Physics and BioQuant,Heidelberg University, 3Institute for Molecular Systems Engineering (IMSE),Heidelberg University

Chapters

  • 00:04Introduction
  • 01:22Preparation of Methacrylated Coverslips
  • 02:39Micropatterning of Extracellular Matrix Proteins on Glass Coverslips
  • 05:31Fabrication of Patterned Polyacrylamide Hydrogels
  • 09:15Local Release of Cell Traction Forces by UV-A Laser Illumination
  • 10:26Image Processing, Particle Image Velocity and Calculation of Traction Forces
  • 10:55Results
  • 11:56Conclusion

Summary

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Near-UV lithography and traction force microscopy are combined to measure cellular forces on micropatterned hydrogels. The targeted light-induced release of single cells enables a high number of measurements on the same sample.

Tags

Hydrogel PatterningTraction Force MicroscopyPolyacrylamide HydrogelMicro-patternExtracellular MatrixFluorescent BeadsCell AdhesionMethacrylated Glass Cover SlipPoly-L-LysineMPEG-SVA

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