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数字液滴PCR法定量分析小鼠视网膜AAV转导效率

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神经科学
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JoVE 杂志神经科学

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

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数字液滴PCR法定量分析小鼠视网膜AAV转导效率

4,196 Views

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09:50 min

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December 25, 2021

DOI:

10.3791/63038-v

DOI:

10.3791/63038-v

•

09:50 min

December 25, 2021

•

14 Views

Iskalen Cansu Topcu Okan^1,2, Mehri Ahmadian^1,2, Yesim Tutuncu^1,2, Halit Yusuf Altay^1,2, Cavit Agca^1,2

¹Molecular Biology, Genetics and Bioengineering Program,Sabanci University, ²Nanotechnology Research and Application Center (SUNUM),Sabanci University

Summary

该协议介绍了如何使用数字液滴PCR（dd-PCR）以及小规模AAV生产，玻璃体内注射，视网膜成像和视网膜基因组DNA分离来量化小鼠视网膜中的AAV转导效率。

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