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Monitoraggio in tempo reale della respirazione mitocondriale in cellule T primarie umane graduate differenziate con citochine
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Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
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Monitoraggio in tempo reale della respirazione mitocondriale in cellule T primarie umane graduate differenziate con citochine

3,516 Views

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06:55 min

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October 19, 2021

DOI:

10.3791/62984-v

DOI:
10.3791/62984-v

•

06:55 min
October 19, 2021

•

8 Views
, , , , , ,
1National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

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Mitochondrial RespirationT CellCytokineSeahorse MachineExtracellular Flux AssayCell-TakXF Cell Culture PlateAssay MediaGlucosePyruvateGlutamine

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Mølgaard, K., Rahbech, A., Met, Ö., Svane, I. M., thor Straten, P., Desler, C., Peeters, M. J. W. Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells. J. Vis. Exp. (176), e62984, doi:10.3791/62984 (2021).

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