Journal
/
/
培养的星形胶质细胞中线粒体系统的实时成像
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Live-imaging of Mitochondrial System in Cultured Astrocytes
DOI:

06:20 min

November 16, 2021

, , ,

Chapters

  • 00:04Introduction
  • 00:44Rat Hippocampal Astrocyte Primary Culture
  • 01:25Long-Term Monitoring of the Mitochondrial System
  • 02:13Analysis of Individual Mitochondrial Morphology and LV-G1-MitoTimer Ratio
  • 03:54Analysis of Mitochondrial Motility
  • 04:28Data Transformation, Normalization, and Statistical Analysis
  • 04:51Results: Analysis of the Effects of H2O2 on the Mitochondrial System of Astrocytes
  • 05:53Conclusion

Summary

自动翻译

本文介绍了配备MitoTimer生物传感器的星形胶质细胞培养物的线粒体延时成像方法,以及由此产生的线粒体动力学,迁移率,形态,生物发生,氧化还原状态和周转的多参数分析。

Related Videos

Read Article