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Live-imaging of Mitochondrial System in Cultured Astrocytes
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Live-imaging of Mitochondrial System in Cultured Astrocytes

Live-imaging of Mitochondrial System in Cultured Astrocytes

3,587 Views

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06:20 min

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November 16, 2021

DOI:

10.3791/62957-v

DOI:
10.3791/62957-v

•

06:20 min
November 16, 2021

•

9 Views
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1Department of Psychiatry, Center for Psychiatric Neurosciences,Lausanne University Hospital (CHUV) and University of Lausanne

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This article describes the method for mitochondrial time-lapse imaging of astrocyte cultures equipped with MitoTimer biosensor and the resulting multiparametric analysis of mitochondrial dynamics, mobility, morphology, biogenesis, redox state, and turnover.

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Espourteille, J., Zufferey, V., Laurent, J., Richetin, K. Live-imaging of Mitochondrial System in Cultured Astrocytes. J. Vis. Exp. (177), e62957, doi:10.3791/62957 (2021).

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