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Live-imaging del sistema mitocondriale in astrociti in coltura
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Live-imaging of Mitochondrial System in Cultured Astrocytes
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Live-imaging del sistema mitocondriale in astrociti in coltura

3,504 Views

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06:20 min

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November 16, 2021

DOI:

10.3791/62957-v

DOI:
10.3791/62957-v

•

06:20 min
November 16, 2021

•

9 Views
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1Department of Psychiatry, Center for Psychiatric Neurosciences,Lausanne University Hospital (CHUV) and University of Lausanne

Summary

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Questo articolo descrive il metodo per l'imaging time-lapse mitocondriale di colture di astrociti dotate di biosensore MitoTimer e la conseguente analisi multiparametrica della dinamica mitocondriale, della mobilità, della morfologia, della biogenesi, dello stato redox e del turnover.

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Cite this Article

Espourteille, J., Zufferey, V., Laurent, J., Richetin, K. Live-imaging of Mitochondrial System in Cultured Astrocytes. J. Vis. Exp. (177), e62957, doi:10.3791/62957 (2021).

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