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多機能システムを用いたヒトがんおよび正常細胞における有形関連タンパク質の全光学機構調査
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December 20, 2021
DOI:
1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering,University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering,University of Florida, 3Department of Electrical and Computer Engineering,University of California, 4Department of Radiation Oncology, College of Medicine,University of Florida, 5UF Health Cancer Center,University of Florida, 6Department of Physics, College of Liberal Arts and Sciences,University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine,University of Florida
Summary
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本稿では、自動マルチチャンネルイメージングとメカノバイオロジカル解析を可能にし、Yes関連タンパク質(YAP)のメカノ感度を解明する統合多機能・ユーザープログラマブルシステムの活用方法に関する詳細な段階的プロトコルを提示する。
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Cite this Article
Luo, Q., Huang, M., Liang, C., Zhang, J., Lin, G., Yu, S., Tanaka, M., Lepler, S., Guan, J., Siemann, D., Tang, X. All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System. J. Vis. Exp. (178), e62934, doi:10.3791/62934 (2021).
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