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Rein optische Mechanobiologie Befragung von Yes-assoziiertem Protein in menschlichen Krebs- und normalen Zellen mit einem multifunktionalen System
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December 20, 2021
DOI:
1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering,University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering,University of Florida, 3Department of Electrical and Computer Engineering,University of California, 4Department of Radiation Oncology, College of Medicine,University of Florida, 5UF Health Cancer Center,University of Florida, 6Department of Physics, College of Liberal Arts and Sciences,University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine,University of Florida
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Dieser Artikel stellt ein detailliertes schrittweises Protokoll zur Verwendung eines integrierten multifunktionalen und benutzerprogrammierbaren Systems vor, das eine automatische Mehrkanalbildgebung und mechanobiologische Analyse ermöglicht, um die Mechanosensitivität des Yes-assoziierten Proteins (YAP) aufzuklären.
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Cite this Article
Luo, Q., Huang, M., Liang, C., Zhang, J., Lin, G., Yu, S., Tanaka, M., Lepler, S., Guan, J., Siemann, D., Tang, X. All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System. J. Vis. Exp. (178), e62934, doi:10.3791/62934 (2021).
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