需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
3,349 Views
•
05:29 min
•
June 09, 2021
DOI:
Summary
Automatically generated
A headspace solid-phase microextraction-gas-chromatography platform is described here for fast, reliable, and semi-automated volatile identification and quantification in ripe blackcurrant fruits. This technique can be used to increase knowledge about fruit aroma and to select cultivars with enhanced flavor for the purpose of breeding.
Explore More Videos
Volatile CompoundsBlackcurrant FruitHeadspace Solid-phase MicroextractionGas Chromatography-mass SpectrometryFruit AromaBreeding ProgramsSemi-automated TechniqueBerry CropsSodium Chloride SolutionCentrifugationInternal StandardGC-MS Auto SamplerHeadspace VialSPME DeviceVolatile DesorptionGC-MS AnalysisRetention TimeMass SpectraKovats Linear Retention IndexPeak AreaInternal StandardBatch Effect Correction
Read Article
Cite this Article
Pott, D. M., Vallarino, J. G., Osorio, S. Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry. J. Vis. Exp. (172), e62421, doi:10.3791/62421 (2021).
Copy