Journal
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发育生物学
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A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

DOI:
10.3791/62318-v

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08:06 min

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March 19, 2021

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, ,
1Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht

Chapters

  • 00:04Introduction
  • 00:50Generation of the Chip
  • 02:21Preparing Microfluidics Experiment
  • 03:44Microfluidics Experiment
  • 05:57Results: Entrainment Analysis Using Microfluidics Experiments
  • 06:58Conclusion

Summary

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A protocol for the culture and manipulation of mouse embryonic tissue on a microfluidic chip is provided. By applying pulses of pathway modulators, this system can be used to externally control signaling oscillations for the functional investigation of mouse somitogenesis.

Tags

MicrofluidicsSignaling OscillationsSomitogenesisSignaling DynamicsPDMSPlasma BondingFibronectin CoatingMouse EmbryosFunctional Dissection

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