Journal
/
发育生物学
/
Elektroporation-medieret RNA interferens metode i Odonata
JoVE 杂志
发育生物学
Author Produced
This content is Free Access.
JoVE 杂志 发育生物学
Electroporation-mediated RNA Interference Method in Odonata

Elektroporation-medieret RNA interferens metode i Odonata

DOI:
10.3791/61952-v

•

13:28 min

•

February 06, 2021

•

, ,
1Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 2Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology (AIST), 3Graduate School of Life and Environmental Sciences,University of Tsukuba

Chapters

  • 00:00Introduction
  • 01:01Protocol: Electroporation-mediated RNAi- In the Abdomen of Damselfly (Ischnura senegalensis)
  • 04:21Protocol: Electroporation-mediated RNAi- In the Thorax of Damselfly (Ischnura senegalensis)
  • 06:54Protocol: Electroporation-mediated RNAi- In the Abdomen of Dragonfly (Pseudothemis zonata)
  • 10:16Representative Results
  • 11:51Discussion

Summary

自动翻译

自动翻译

Vi leverer en detaljeret protokol for elektroporation-medieret RNA interferens i insekter af ordren Odonata (guldsmede og damselflies) ved hjælp af blå-tailed damselfly (Ischnura senegalensis: Coenagironidae: Zygoptera) og pied skiflue guldsmed guldsmed (Pseudothemis zonata: Libellulidae: Anptisoera).

Tags

Keywords: ElectroporationRNA InterferenceRNAiOdonataDragonflyDamselflyIschnura SenegalensisGene Function AnalysisIn VivoProthoraxAbdomenSiRNADsRNAElectroporator

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/61952?article_type=v

Transkriptionel Analyse af Nascent RNA FISH af<em> In vivo</em> Trofoblasten kæmpeceller eller<em> In vitro</em> Kortsigtede Kulturer af Ectoplacental Cone Eksplantater
check_url/cn/61952?article_type=v

Instrueret differentiering af Primitive og endelige hæmatopoietisk ophav fra humane pluripotente stamceller
check_url/cn/61952?article_type=v

En metode til kendetegner embryogenese i Arabidopsis
check_url/cn/61952?article_type=v

Dissektion og immunfluorescent farvning af champignon krop og fotoreceptor neuroner i voksen Drosophila melanogaster hjerner
check_url/cn/61952?article_type=v

Visualisere Node og Notochordal plade i Gastrulating mus embryoner ved hjælp af Scanning elektronmikroskopi og hele Mount immunfluorescens
check_url/cn/61952?article_type=v

En In Vivo -metode til at studere mus blod-Testis barriere integritet
check_url/cn/61952?article_type=v

RNA-baserede omprogrammering af menneskelige primære fibroblaster til induceret pluripotente stamceller
check_url/cn/61952?article_type=v

Dissektion af Drosophila puppe nethinden for immunhistokemi, vestlige analyse og RNA isolering
check_url/cn/61952?article_type=v

Elektro porationsmetode til in vivo-levering af plasmid-DNA i den voksne Zebrafish telencephalon
check_url/cn/61952?article_type=v

En co-kultur metode til at studere Neurite Udvækst som reaktion på Dental Pulp Paracrine Signaler

Read Article