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レーザーフリーヒドロキシルラジカルタンパク質フットプリントによるタンパク質の高次構造解析
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生物化学
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JoVE 杂志 生物化学
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins
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レーザーフリーヒドロキシルラジカルタンパク質フットプリントによるタンパク質の高次構造解析

3,444 Views

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09:59 min

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June 04, 2021

DOI:

10.3791/61861-v

DOI:
10.3791/61861-v

•

09:59 min
June 04, 2021

•

1 Views
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1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy,University of Mississippi, 3Department of Chemistry and Biochemistry,University of Mississippi

Tags

Protein FootprintingHydroxyl RadicalLaser-freeProtein StructureProtein InteractionProtein AggregationProtein StabilityCapillaryPhotolysisDosimetrySilicaPolyimideNutFerrulePhotolysis CellDosimeter CellMagnetic MaskTeflon TubingInjection Loop

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Cite this Article

Weinberger, S. R., Chea, E. E., Sharp, J. S., Misra, S. K. Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins. J. Vis. Exp. (172), e61861, doi:10.3791/61861 (2021).

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