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将多重荧光原位杂交与荧光免疫组化相结合，对新鲜冷冻或固定的小鼠脑切片进行研究

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神经科学

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JoVE 杂志神经科学

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

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将多重荧光原位杂交与荧光免疫组化相结合，对新鲜冷冻或固定的小鼠脑切片进行研究

5,744 Views

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07:36 min

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June 25, 2021

DOI:

10.3791/61709-v

DOI:

10.3791/61709-v

•

07:36 min

June 25, 2021

•

30 Views

Ayse S. Dereli¹, Evan J. Bailey¹, Natasha N. Kumar¹

¹Department of Pharmacology, School of Medical Sciences,University of New South Wales

Summary

该协议描述了一种在新鲜冷冻和固定小鼠脑切片中结合荧光原位杂交（FISH）和荧光免疫组织化学（IHC）的方法，目的是实现多标记FISH和荧光IHC信号。IHC 靶向细胞质和膜附着蛋白。

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