Journal
/
/
Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

DOI:

07:28 min

October 29, 2020

, , ,

Chapters

  • 00:04Introduction
  • 00:42Sample Preparation
  • 01:20Insert Preparation and Loading
  • 02:10Sample Analysis
  • 03:37Data Analysis
  • 05:07Results: Representative Antibiotic Effects on A. baumannii DSM-30008 Growth and Metabolism
  • 06:59Conclusion

Summary

自动翻译

The elucidation of the mode-of-action of a novel antibiotic is a challenging task in the drug discovery process. The goal of the method described here is the application of isothermal microcalorimetry using calScreener in antibacterial profiling to provide additional insight into drug-microbe interactions.

Related Videos

Read Article