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Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

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神经科学

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JoVE 杂志神经科学

Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

3,384 Views

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06:21 min

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October 06, 2020

DOI:

10.3791/61662-v

DOI:

10.3791/61662-v

•

06:21 min

October 06, 2020

•

3 Views

David G. Keener*^1,2, Amy Cheung*^1,3, Kensuke Futai

¹Brudnick Neuropsychiatric Research Institute, Department of Neurobiology,University of Massachusetts Medical School, ²Interdisciplinary Graduate Program,University of Massachusetts Medical School, ³UMMS MD/PhD Program,University of Massachusetts Medical School

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Single-cell Electroporation Organotypic Slice Culture Mouse Hippocampus Excitatory Neurons Inhibitory Neurons Gene Transfection Neurobiology Electroporation Protocol High Transfection Efficiency Cell-autonomous Mechanisms Transsynaptic Protein Interactions Slice Culture Preparation Electroporator Setup Pipette Preparation Slice Culture Isolation Target Cell Electroporation

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