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Detección de variantes genéticas en el gen CALR mediante análisis de fusión de alta resolución
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Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
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Detección de variantes genéticas en el gen CALR mediante análisis de fusión de alta resolución

4,366 Views

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08:46 min

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August 26, 2020

DOI:

10.3791/61642-v

DOI:
10.3791/61642-v

•

08:46 min
August 26, 2020

•

3 Views
, , , , , ,
1Department of Hematology,University Medical Centre Ljubljana, 2Clinical Institute for Genomic Medicine,University Medical Centre Ljubljana, 3Faculty of Pharmacy,University of Ljubljana

Tags

Genetic Variant DetectionCALR GeneHigh Resolution Melting AnalysisMyeloproliferative DisordersEssential ThrombocythemiaPrimary MyelofibrosisQPCR HRM Master MixNegative ControlsPositive ControlsNo Template Control (NTC)Optical Adhesive FilmDissociation AnalysisAmplification PlotFluorescence ReadingsPassive Reference

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Pajič, T., Belčič Mikič, T., Podgornik, H., Klun, J., Šućurović, S., Zver, S., Sever, M. Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis. J. Vis. Exp. (162), e61642, doi:10.3791/61642 (2020).

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