Journal
/
癌症研究
/
A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
JoVE 杂志
癌症研究
This content is Free Access.
JoVE 杂志 癌症研究
A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties

A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties

5,878 Views

•

08:40 min

•

August 07, 2020

DOI:

10.3791/61606-v

DOI:
10.3791/61606-v

•

08:40 min
August 07, 2020

•

13 Views
, , , , ,
1Department of Medical Cell Biology,Uppsala University, 2Polymer Chemistry, Department of Chemistry-Ångström Laboratory,Uppsala University

Summary

Automatically generated

This protocol presents a 3D biomimetic model with accompanying fibrotic stromal compartment. Prepared with physiologically relevant hydrogels in ratios mimicking the bio-physical properties of the stromal extracellular matrix, an active mediator of cellular interactions, tumor growth and metastasis.

Explore More Videos

3D Tumor ModelBiomimeticTumor-stroma InteractionsLiver CancerHydrogelCell CultureCollagen CoatingFibrinogenCell LinesHepatocellular CarcinomaMicroenvironment

Read Article

Cite this Article

Calitz, C., Pavlović, N., Rosenquist, J., Zagami, C., Samanta, A., Heindryckx, F. A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties. J. Vis. Exp. (162), e61606, doi:10.3791/61606 (2020).

Download .ris file

Copy

Share Video

.

Copy