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荧光染料标记脂质体体内组织特异性细胞摄取的制备、给药和评估

JoVE 杂志
生物工程

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JoVE 杂志生物工程

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

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荧光染料标记脂质体体内组织特异性细胞摄取的制备、给药和评估

3,226 Views

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08:44 min

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July 30, 2020

DOI:

10.3791/61585-v

DOI:

10.3791/61585-v

•

08:44 min

July 30, 2020

•

44 Views

Victoria Osinski^1,2, Alexander L Klibanov^1,3, Coleen A McNamara^1,3

¹Robert M. Berne Cardiovascular Research Center,University of Virginia, ²Department of Pathology,University of Virginia, ³Department of Medicine, Division of Cardiovascular Medicine,University of Virginia

Summary

该协议的目标是合成荧光标记的脂质体，并使用流式细胞术在细胞水平上鉴定脂质体的体内定位。

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