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成人斑马鱼中西斯铂诱导的急性肾损伤模型
JoVE 杂志
医学
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JoVE 杂志 医学
Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish
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成人斑马鱼中西斯铂诱导的急性肾损伤模型

5,077 Views

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13:25 min

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May 15, 2021

DOI:

10.3791/61575-v

DOI:
10.3791/61575-v

•

13:25 min
May 15, 2021

•

7 Views
, , , , , ,
1Department of Immunology,University of São Paulo, 2Department of Medicine, Nephrology Division,Federal University of São Paulo

Summary

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本协议描述了使用西斯铂作为肾毒剂在成年斑马鱼中诱导急性肾损伤 （AKI） 的程序。我们详细介绍了评估该技术和两种技术的可重复性，分别分析肾脏组织、流动细胞学和TUNEL中的炎症和细胞死亡。

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Morales Fénero, C., Padovani, B. N., do Amaral, M. A., de Barros, G. J. B., de Oliveira, I. K. X., Hiyane, M. I., Camâra, N. O. S. Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish. J. Vis. Exp. (171), e61575, doi:10.3791/61575 (2021).

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