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通过流细胞学测定,测量自然获得的噬菌体诱导恶性疟Plasmodium falciparum原虫抗体
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay
DOI:

09:57 min

August 06, 2020

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Chapters

  • 00:04Introduction
  • 00:33THP-1 and Infected Erythrocyte (IE) Culture Setup
  • 01:58Parasite Staining and Opsonization Plate Preparation
  • 03:47THP-1 Cell and Phagocytosis Plate Preparation
  • 04:35Phagocytosis Assay
  • 06:21Flow Cytometry Acquisition and Analysis
  • 07:34Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
  • 09:21Conclusion

Summary

自动翻译

该协议的总体目标是指导如何测量自然接触恶性疟原虫感染的血清或血浆中的抗体的能力,以对抗和诱导寄生虫感染的红细胞(IEs)的噬菌体。 Plasmodium falciparum

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