需要订阅 JoVE 才能查看此. 登录或开始免费试用。
1Department of Plant Biology,Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences,Kyoto University, 3Department of Biochemistry,Purdue University, 4Department of Chemistry,Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences
Chapters
- 00:04Introduction
- 00:33Immobilized Metal Ion Affinity Chromatography (IMAC) and High pH Reversed-Phase (Hp-RP) Stage Tip Preparation
- 01:10IMAC Stage Tip Phosphopeptide Enrichment
- 02:44Hp-RP C18 Stage Tip Phosphopeptide Fractionation
- 03:37Results: Representative Osmotic Stress Signaling in Arabidopsis
- 05:05Conclusion
Qui è presentato un approccio fosfoproteomico, vale a dire fosfoproteomico a base di punta di estrazione stop and go, che fornisce un'elevata produttività e una copertura profonda del fosfoproteoma arabidopsis. Questo approccio delinea la panoramica della segnalazione dello stress osmotico in Arabidopsis.
