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Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

DOI:
10.3791/61309-v

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04:32 min

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October 23, 2020

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, , , , , , , , ,
1Department of Anesthesiology and Critical Care, Soroka Medical Center,Ben-Gurion University of the Negev, 2Department of Anesthesiology,Yale University School of Medicine, 3Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev, 4Department of Physiology, Faculty of Biology, Ecology and Medicine,Dnepropetrovsk State University

Chapters

  • 00:04Introduction
  • 00:54Determination of Infarct Zone and Brain Edema
  • 02:37Determination of BBB Disruption
  • 03:23Results: Histological Outcomes of MCAO Rats Compared to Sham-operated Rats
  • 03:57Conclusion

Summary

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This protocol describes a novel technique of measuring the three most important parameters of ischemic brain injury on the same set of rodent brain samples. Using only one brain sample is highly advantageous in terms of ethical and economic costs.

Tags

Keywords: StrokeCerebral EdemaInfarct ZoneBlood-brain BarrierBBB PermeabilityMiddle Cerebral Artery OcclusionIschemic Brain InjuryTTC StainingImageJInfarct VolumeBrain EdemaTrichloroacetic AcidLuminescence Spectrophotometry

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