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使用亚细胞室特异性红氧敏感绿色荧光蛋白评估细胞氧化
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生物化学
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Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
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使用亚细胞室特异性红氧敏感绿色荧光蛋白评估细胞氧化

7,065 Views

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06:10 min

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June 18, 2020

DOI:

10.3791/61229-v

DOI:
10.3791/61229-v

•

06:10 min
June 18, 2020

•

4 Views
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1Division of Radiation Health, Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy,Ege University

Summary

Automatically generated

该协议描述了对细胞内亚细胞室特异性氧化还原状态的评估。对氧化还原敏感的荧光探针可在完整细胞中方便地进行比例分析。

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Cite this Article

Tascioglu Aliyev, A., LoBianco, F., Krager, K. J., Aykin-Burns, N. Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein. J. Vis. Exp. (160), e61229, doi:10.3791/61229 (2020).

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