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高通量酵母菌株，带基于滴滴的RNA测序

JoVE 杂志
生物工程

This content is Free Access.

JoVE 杂志生物工程

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

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高通量酵母菌株，带基于滴滴的RNA测序

6,627 Views

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07:55 min

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May 21, 2020

DOI:

10.3791/61014-v

DOI:

10.3791/61014-v

•

07:55 min

May 21, 2020

•

2 Views

Jesse Q. Zhang^1,2, Kai-Chun Chang¹, Leqian Liu¹, Zev J. Gartner^3,5, Adam R. Abate^1,4,5

¹Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, ²University of California Berkeley-UCSF Graduate Program in Bioengineering,University of California San Francisco, ³Department of Pharmaceutical Chemistry,University of California San Francisco, ⁴California Institute for Quantitative Biosciences,University of California San Francisco, ⁵Chan Zuckerberg Biohub

Summary

微生物工程"设计-构建-测试"周期的一个瓶颈是，我们可以执行应变功能屏幕的速度。我们描述了一种高通量方法，用于菌株筛选，应用于每个实验的数百至数千个酵母细胞，利用基于滴滴的RNA测序。

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