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基于AFM的力光谱单分子共价附价
JoVE 杂志
化学
This content is Free Access.
JoVE 杂志 化学
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
DOI:

10:37 min

March 16, 2020

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Chapters

  • 00:04Introduction
  • 00:52Surface Activation, Silanization, and PEGylation
  • 03:03Covalent Polymer Attachment
  • 04:21Determining the Inverse Optical Lever Sensitivity (InVOLS)
  • 06:13Determining the AFM Cantilever Force Constant
  • 07:06Data Collection
  • 08:10Results: Single-Molecule Stretching and Desorption
  • 09:44Conclusion

Summary

自动翻译

探针分子附于原子力显微镜(AFM)悬臂尖端是研究其物理特性的关键技术。这使我们能够通过基于 AFM 的单分子力光谱,确定聚合物的拉伸力、解吸力和长度,具有高可重复性。

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