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雄性小鼠生殖细胞中的核糖核菌免疫沉淀
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
DOI:

10:00 min

March 04, 2020

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Chapters

  • 00:05Introduction
  • 00:56Preparation of Solutions
  • 01:57Preparation of Tissue
  • 02:49Homogenization of Sample
  • 04:03Equilibration of Beads
  • 04:59Preclearing Sample
  • 05:56Incubation of Antibody and Beads
  • 06:29Washing of Beads
  • 07:21RNA Extraction
  • 07:54Results: Negative Controls, Reagents Impact, and a Mutant Model
  • 09:09Conclusion

Summary

自动翻译

在这里,我们描述了核糖体和相关RNA的免疫沉淀从成年雄性小鼠生殖细胞的精选群体使用RiboTag系统。战略性育种和精心的免疫沉淀产生清洁、可重复的结果,为生殖细胞的翻译提供信息,并深入了解突变表型的机制。

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