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Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
JoVE 杂志
医学
This content is Free Access.
JoVE 杂志 医学
Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

DOI:
10.3791/60776-v

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07:40 min

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March 20, 2021

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, , , , , , ,
1Translational Medicine, R&D Oncology,AstraZeneca, 2Acerta Pharma, AstraZeneca Group, 3Translational Medicine, R&D Oncology,AstraZeneca

Chapters

  • 00:05Introduction
  • 01:08Whole Blood Irradiation for DNA Damage Response (DDR) Biomarker Expression Analysis
  • 03:35PBMC and Plasma Freezing
  • 04:45Western Blot Analysis
  • 05:43Results: Representative DNA Damage Assessment
  • 07:10Conclusion

Summary

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This protocol details clinically implementable preparation of high quality PBMC and plasma biosamples at the clinical trial site that can be used for translational biomarker analysis.

Tags

PBMCPlasmaBlood DrawBiomarker AnalysisClinical TrialPre-analytical VariabilityPharmacokineticsPharmacodynamicsDNA Damage ResponseRadiationCentrifugationCell SeparationBuffy Coat

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