Here we present a screening method for membrane-bound pyrophosphatase (from Thermotoga maritima) inhibitors based on the molybdenum blue reaction in a 96 well plate format.
Vidilaseris, K., Johansson, N. G., Turku, A., Kiriazis, A., Boije af Gennäs, G., Yli-Kauhaluoma, J., Xhaard, H., Goldman, A. Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors. J. Vis. Exp. (153), e60619, doi:10.3791/60619 (2019).