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Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
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November 23, 2019
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1Research Program in Molecular and Integrative Biosciences,University of Helsinki, 2Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy,University of Helsinki, 3School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology,University of Leeds
Summary
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Here we present a screening method for membrane-bound pyrophosphatase (from Thermotoga maritima) inhibitors based on the molybdenum blue reaction in a 96 well plate format.
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Vidilaseris, K., Johansson, N. G., Turku, A., Kiriazis, A., Boije af Gennäs, G., Yli-Kauhaluoma, J., Xhaard, H., Goldman, A. Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors. J. Vis. Exp. (153), e60619, doi:10.3791/60619 (2019).
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