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Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds
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JoVE 杂志 环境科学
Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

DOI:
10.3791/60462-v

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12:02 min

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August 08, 2020

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, , , , , , , , ,
1Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences,Szent István University, 2Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences,University of Birmingham

Chapters

  • 00:00Introduction
  • 02:52Embryon Harvest and Treatment
  • 05:05Larvae Preparation fo Photography
  • 06:05Microscopy
  • 07:02Determining Integrated Density
  • 08:14Representative Results
  • 10:20Conclusions

Summary

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Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).

Tags

Tg(Vtg1:mcherry) ZebrafishEndocrine Disrupting CompoundsEstrogenic EffectsBiomarkerVitellogeninIn Vivo TestingEnvironmental SamplesAnimal Welfare

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