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In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes
JoVE 杂志
医学
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JoVE 杂志 医学
In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

5,490 Views

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08:19 min

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June 22, 2020

DOI:

10.3791/60427-v

DOI:
10.3791/60427-v

•

08:19 min
June 22, 2020

•

6 Views
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1Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina,Universidade do Porto, 2Department of Physiology,Kilimanjaro Christian Medical University College

Summary

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This protocol aims to describe step-by-step the technique of extraction and assessment of cardiac function using skinned cardiomyocytes. This methodology allows measurement and acutemodulation of myofilament function using small frozen biopsies that can be collected from different cardiac locations, from mice to men.

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Cite this Article

Gonçalves-Rodrigues, P., Almeida-Coelho, J., Gonçalves, A., Amorim, F., Leite-Moreira, A. F., Stienen, G. J., Falcão-Pires, I. In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes. J. Vis. Exp. (160), e60427, doi:10.3791/60427 (2020).

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